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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Fluorescence intensity decay shape microscopy ： ウィキペディア英語版 Fluorescence intensity decay shape microscopy Fluorescence intensity decay shape microscopy (FIDSAM) is a fluorescence microscopy technique, which utilizes the time evolution of fluorescence emission after a pulsed excitation to analyse the decay statistics of an excited chromophore. The main application of FIDSAM is the discrimination of unspecific autofluorescent background signal from the target signal of a dedicated chromophore. == Principle == The FIDSAM method analyses the number of different molecules contributing to a measured fluorescence signal. Assuming a pure fluorescent dye solution in an isotropic surrounding, the individual emitters are indistinguishable. Accordingly, they obey the same fluorescence emission statistics and the time evolution of the fluorescence emission after a pulsed excitation can be described by a monoexponential decay function according to: :$F(t)\; =\; A\; e^$ with $A$ = the initial fluorescence intensity after the excitation and $\backslash tau$ = the decay constant (fluorescence lifetime). In contrast, autofluorescent background consists of a multitude of individual emitters, which obey individual emission statistics. Accordingly, the time evolution samples a summation of numerous individual decay statistics an can be written as: :$F(t)\; =\; \backslash sum(A\_1e^)$. The FIDSAM technique bases on a time correlated single photon counting (TCSPC) measurement and analyses the degree of deviation of a recorded fluorescence decay from a monoexponential behavior. This is achieved by fitting the recorded fluorescence intensity decay by a monoexponential decay function convoluted with the instrument response function. In a next step, the error value of the fitting procedure, $\backslash chi^2$, is extracted and its inverse value is multiplied with the original intensity value. This way, fluorescence signal, which originates from autofluorescence background and therefore exhibits increased error-values, is divided by a relatively large number, whereas fluorescence signal from target molecules exhibits small error-values around unity is divided by a small number and remains largely unaffected. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Fluorescence intensity decay shape microscopy」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.